Polymorphic microsatellite markers for population analysis of a tephritid pest species, Bactrocera tryoni.

@article{Kinnear1998PolymorphicMM,
  title={Polymorphic microsatellite markers for population analysis of a tephritid pest species, Bactrocera tryoni.},
  author={M W Kinnear and Harbans Bariana and John A. Sved and Marianne Frommer},
  journal={Molecular ecology},
  year={1998},
  volume={7 11},
  pages={1489-95}
}
To obtain a set of microsatellite markers for the Queensland fruit fly Bactrocera tryoni, a genomic library was screened with a number of simple repeat oligonucleotide probes. Sequencing recovered 22 repeat loci. The microsatellite sequences were short, with repeat numbers ranging from five to 11. Of these, 16 polymerase chain reaction (PCR) primer sets yielded amplifiable products, which were tested on 53 flies from five widely separated sites. All loci showed polymorphism in the population… CONTINUE READING
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