Polymorphic Nucleotide within the Promoter of Nitrate Reductase (NarGHJI) Is Specific for Mycobacterium tuberculosis

@article{Stermann2003PolymorphicNW,
  title={Polymorphic Nucleotide within the Promoter of Nitrate Reductase (NarGHJI) Is Specific for Mycobacterium tuberculosis},
  author={Marion Stermann and Antje Bohrssen and Catharina Diephaus and Silvia Maass and Franz-Christoph Bange},
  journal={Journal of Clinical Microbiology},
  year={2003},
  volume={41},
  pages={3252 - 3259}
}
ABSTRACT Mycobacterium tuberculosis rapidly reduces nitrate, leading to the accumulation of nitrite. This characteristic served for the past 40 years to differentiate M. tuberculosis from other members of the Mycobacterium tuberculosis complex (MTBC), such as Mycobacterium bovis (non-BCG [referred to here as simply “M. bovis”]), Mycobacterium bovis BCG, Mycobacterium africanum, or Mycobacterium microti. Here, a narG deletion in M. tuberculosis showed that rapid nitrite accumulation of M… 
A Promoter Mutation Causes Differential Nitrate Reductase Activity of Mycobacterium tuberculosis and Mycobacterium bovis
TLDR
A genetic approach is used to demonstrate that an SNP within the promoter of the nitrate reductase gene cluster narGHJI is responsible for the different nitrate reduction activity of M. tuberculosis and M. bovis.
Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2.
TLDR
Regulation of both the nitrate reductase enzyme and transporter are regulated differently in the two species.
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It is concluded that NarGHJI and NirBD of M. tuberculosis mediate the assimilatory reduction of nitrate and nitrite, respectively, and that GlnR acts as a transcriptional activator of nirBD.
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Potential genes involved in regulating the nitrate reductase expression in M. tuberculosis were identified and the conserved NarG might be an alternative mycobacterium taxonomic marker.
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Systems-Based Approaches to Probing Metabolic Variation within the Mycobacterium tuberculosis Complex
TLDR
In this study genome scale metabolic networks of M. bovis and M.bovis BCG were constructed and interrogated, along with a M. tuberculosis network, to predict substrate utilisation, gene essentiality and growth rates, and successfully simulate many aspects of the growth and physiology of these mycobacteria.
Comparative ‘omics analyses differentiate Mycobacterium tuberculosis and Mycobacterium bovis and reveal distinct macrophage responses to infection with the human and bovine tubercle bacilli
TLDR
The work presented here provides a basis for the identification of host innate immune mechanisms subverted by virulent host-adapted mycobacteria to promote their survival during the early stages of infection.
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