Polymerization properties of the Thermotoga maritima actin MreB: roles of temperature, nucleotides, and ions.

Abstract

MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its cellular functions, we know very little about the biochemical behavior of MreB. We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from Thermotoga maritima. We have… (More)

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