Polymerization of an Escherichia coli elongation factor Tu.

  title={Polymerization of an Escherichia coli elongation factor Tu.},
  author={Michael K Helms and David Jameson},
  journal={Archives of biochemistry and biophysics},
  volume={321 2},
Elongation factor Tu (EF-Tu) from Escherichia coli is shown here to polymerize under conditions of low ionic strength and slightly acidic pH. Several factors, such as decreasing pH, decreasing ionic strength, increasing temperature, and increasing protein concentration (up to 2.5 microM) enhance the rate of polymerization. Both EF-Tu.GTP and EF-Tu.GDP… CONTINUE READING