Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy.

  title={Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy.},
  author={Ikuko Fujiwara and Dimitrios Vavylonis and Thomas D. Pollard},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={104 21},
We used fluorescence microscopy to determine how polymerization of Mg-ADP-actin depends on the concentration of phosphate. From the dependence of the elongation rate on the actin concentration and direct observations of depolymerizing filaments, we measured the polymerization rate constants of ADP-actin and ADP-P(i)-actin. Saturating phosphate reduces the critical concentration for polymerization of Mg-ADP-actin from 1.8 to 0.06 microM almost entirely by reducing the dissociation rate constants… CONTINUE READING


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