Polymerization and mechanical properties of single RecA-DNA filaments.

@article{Hegner1999PolymerizationAM,
  title={Polymerization and mechanical properties of single RecA-DNA filaments.},
  author={Martin Hegner and Steven R. B. Smith and Carlos Bustamante},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 18},
  pages={
          10109-14
        }
}
The polymerization of individual RecA-DNA filaments, containing either single-stranded or double-stranded DNA, was followed in real time, and their mechanical properties were characterized with force-measuring laser tweezers. It was found that the stretch modulus of a filament is dominated by its (central) DNA component, while its bending rigidity is controlled by its (eccentric) protein component. The longitudinal stiffness of DNA increases 6- to 12-fold when the DNA is contained in the… CONTINUE READING

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