Polymerase resistance to polymerase chain reaction inhibitors in bone*.

@article{Eilert2009PolymeraseRT,
  title={Polymerase resistance to polymerase chain reaction inhibitors in bone*.},
  author={Kenneth D Eilert and David R. Foran},
  journal={Journal of forensic sciences},
  year={2009},
  volume={54 5},
  pages={1001-7}
}
Amplification of DNA from aged or degraded skeletal remains can be a challenging task, in part due to naturally occurring inhibitors of the polymerase chain reaction. PCR inhibitors may act by inactivating a polymerase itself, or compete with or bind other reaction components, although various polymerases may be differentially susceptible to such insult. In this study, ten thermostable polymerases from six bacterial species were examined for their ability to amplify DNA in the presence of bone… CONTINUE READING