Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates.

@article{Schwarz1996PolymeraseCR,
  title={Polymerase chain reaction for diagnosis and identification of distinct variants of Crimean-Congo hemorrhagic fever virus in the United Arab Emirates.},
  author={Tino F Schwarz and Herbert N Nsanze and M R Longson and H. M. Nitschko and Sabine Gilch and H H Shurie and Ahmadul Ameen and Allan Knies Hans Mulder Rumi Zahir and Usha Acharya and Gerry Jager},
  journal={The American journal of tropical medicine and hygiene},
  year={1996},
  volume={55 2},
  pages={190-6}
}
Viral hemorrhagic fever has re-emerged in the United Arab Emirates (UAE) since November 1993. Genomic RNA of Crimean-Congo hemorrhagic virus (C-CHFV) was detected by a newly developed, nested reverse transcriptase polymerase chain reaction (RT-PCR) in the sera of four (25.0%) of 16 suspected cases of viral hemorrhagic fever. The RT-PCR was based on oligonucleotide primers deducted from the small RNA segment encoding the nucleoprotein of the virus. By comparison with a nucleotide sequence of a C… CONTINUE READING

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