Polymerase chain reaction for detection of verocytotoxigenic Escherichia coli isolated from animal and food sources.

@article{Read1992PolymeraseCR,
  title={Polymerase chain reaction for detection of verocytotoxigenic Escherichia coli isolated from animal and food sources.},
  author={Susan C Read and Rachael Clarke and Annett Martin and S A De Grandis and Jeffery L Hii and Scott A. McEwen and Carlton L. Gyles},
  journal={Molecular and cellular probes},
  year={1992},
  volume={6 2},
  pages={153-61}
}
Animals and their by-products have been implicated as important sources of verocytotoxigenic Escherichia coli (VTEC) associated with disease in humans. VTEC comprise a wide range of serotypes and produce a variety of closely related verocytotoxins (VT). A pair of oligonucleotide primers, targeting conserved sequences found in VT1, VT2 and VTE genes, was used to develop a polymerase chain reaction (PCR) procedure to detect all types of VTEC. Supernatants of boiled broth cultures of VTEC (223… CONTINUE READING
16 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Similar Papers

Loading similar papers…