Polymerase chain reaction detection of nonviable bacterial pathogens.

  title={Polymerase chain reaction detection of nonviable bacterial pathogens.},
  author={Karen L. Josephson and Charles P Gerba and Ian L. Pepper},
  journal={Applied and environmental microbiology},
  volume={59 10},
Polymerase chain reaction (PCR) methodologies for detection of pathogens in environmental samples are currently available. However, positive amplification products for any set of primers only signal that the appropriate target nucleic acid sequences were present in the sample. The presence of the amplification products does not imply that the target organisms were viable. Here we show that PCR will detect nonviable cells, as long as intact target nucleic acid sequences are available. In an… CONTINUE READING


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