Polymerase chain reaction-based prevalence of hepatitis A, hepatitis E and TT viruses in sewage from an endemic area.

@article{Vaidya2002PolymeraseCR,
  title={Polymerase chain reaction-based prevalence of hepatitis A, hepatitis E and TT viruses in sewage from an endemic area.},
  author={Sunil R Vaidya and Shobha D Chitambar and Vidya A Arankalle},
  journal={Journal of hepatology},
  year={2002},
  volume={37 1},
  pages={131-6}
}
BACKGROUND/AIMS Hepatitis A and E viruses (HAV, HEV) are transmitted enterically and are highly endemic in India. This study aims to evaluate prevalence of these and TT virus (TTV) in the sewage. METHODS Influent and effluent samples from a sewage treatment plant from Pune, India were collected twice a week for 1 year and subjected to nested polymerase chain reaction (PCR) for the detection of HAV RNA, HEV RNA and TTV DNA. HAV and HEV PCR products were sequenced. Effluent samples were not… CONTINUE READING

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