Polymerase chain reaction-based differentiation of the mosquito sibling species Anopheles claviger s.s. and Anopheles petragnani (Diptera: Culicidae).

@article{Kampen2003PolymeraseCR,
  title={Polymerase chain reaction-based differentiation of the mosquito sibling species Anopheles claviger s.s. and Anopheles petragnani (Diptera: Culicidae).},
  author={Helge Kampen and Anja Sternberg and J. de Proft and Sandra Bastian and Francis Schaffner and Walter A. Maier and Hanns Martin Seitz},
  journal={The American journal of tropical medicine and hygiene},
  year={2003},
  volume={69 2},
  pages={
          195-9
        }
}
A polymerase chain reaction (PCR)-based diagnostic assay was developed that rapidly and reliably differentiates the sibling species of the Anopheles claviger complex, An. claviger s.s. and An. petragnani. The assay makes use of nucleotide differences in the internal transcribed spacer 2 ribosomal DNA sequences to generate PCR products of specific length for each of the two species. In evaluating the test, 580 of 592 field-collected An. claviger s.l. specimens were unambiguously identified as… CONTINUE READING

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