Polymerase chain reaction-based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion.

@article{Pitkranta1998PolymeraseCR,
  title={Polymerase chain reaction-based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion.},
  author={Anne Pitk{\"a}ranta and Jussi Jero and Eurico Arruda and Anni Virolainen and Frederick G. Hayden},
  journal={The Journal of pediatrics},
  year={1998},
  volume={133 3},
  pages={390-4}
}
OBJECTIVES To study the association of human rhinovirus (HRV), respiratory syncytial virus (RSV), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (OME). To determine how long HRV RNA can be detected after HRV infection. METHODS Middle ear effusion (MEE) samples collected at the time of tympanostomy tube placement from 100 children with OME were examined. Viral RNA was detected by reverse-transcriptase polymerase chain reaction. For HRV… CONTINUE READING
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