Polymerase chain reaction-based detection of dermatophyte DNA with a fungus-specific primer system.

@article{Bock1994PolymeraseCR,
  title={Polymerase chain reaction-based detection of dermatophyte DNA with a fungus-specific primer system.},
  author={Marison Bock and Matthias Maiwald and Reinhard Kappe and Peter Nickel and Helmut Naeher},
  journal={Mycoses},
  year={1994},
  volume={37 3-4},
  pages={79-84}
}
There is significant clinical interest in primers which are specific for fungi and do not hybridize to DNA of other eukaryotes or prokaryotes. Such primers would allow specific amplification of fungal DNA from human tissue samples containing fungi. Fungal identification to the species level could follow by direct sequencing or restriction analysis. Several previously described primer systems cross-react with DNA of plants and animals. We have designed a primer system that amplifies a fragment… CONTINUE READING

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