Polymerase chain reaction (PCR) for the detection of herpesvirus in tortoises.

@article{Une2000PolymeraseCR,
  title={Polymerase chain reaction (PCR) for the detection of herpesvirus in tortoises.},
  author={Yumi Une and Mariko Murakami and Koichi Uemura and Hideo Fujitani and Toshio Ishibashi and Yasumasa Nomura},
  journal={The Journal of veterinary medical science},
  year={2000},
  volume={62 8},
  pages={
          905-7
        }
}
A polymerase chain reaction (PCR)-based method using a herpesvirus consensus primer was assessed for the identification of herpesviral infections in tortoises. A single band of about 230 bp was detected in PCR products from two out of twenty swabs taken from the oral cavity, three out of three paraffin-embedded tissue sections from the liver (two cases) and oral mucosa (one case), and one out of two fresh tissue samples from the oral mucosa. Nucleotide sequencing of these PCR products indicated… CONTINUE READING

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