Polymer versus monomer as displacer in immobilized metal affinity chromatography.

@article{Arvidsson2001PolymerVM,
  title={Polymer versus monomer as displacer in immobilized metal affinity chromatography.},
  author={P. Arvidsson and A. E. Ivanov and Galaev IYu and B. Mattiasson},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={2001},
  volume={753 2},
  pages={
          279-85
        }
}
  • P. Arvidsson, A. E. Ivanov, +1 author B. Mattiasson
  • Published 2001
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical sciences and applications
  • Successful immobilized metal affinity chromatography (IMAC) of proteins on Cu2+-iminodiacetic acid Sepharose has been carried out in a displacement mode using a synthetic copolymer of vinyl imidazole and vinyl caprolactam [poly(VI-VCL)] as a displacer. Vinyl caprolactam renders the co-polymer with the thermosensitivity, e.g., property of the co-polymer to precipitate nearly quantitatively from aqueous solution on increase of the temperature to 48 degrees C. A thermostable lactate dehydrogenase… CONTINUE READING
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