Polyketide synthase (PKS) reduces fusion of Legionella pneumophila-containing vacuoles with lysosomes and contributes to bacterial competitiveness during infection.

@article{Shevchuk2014PolyketideS,
  title={Polyketide synthase (PKS) reduces fusion of Legionella pneumophila-containing vacuoles with lysosomes and contributes to bacterial competitiveness during infection.},
  author={Olga Shevchuk and Dennis P{\"a}gelow and Janine Rasch and Simon D{\"o}hrmann and Gabriele G{\"u}nther and Julia Hoppe and Can Murat {\"U}nal and Marc Bronietzki and Maximiliano G Gutierrez and Michael Steinert},
  journal={International journal of medical microbiology : IJMM},
  year={2014},
  volume={304 8},
  pages={
          1169-81
        }
}
L. pneumophila-containing vacuoles (LCVs) exclude endocytic and lysosomal markers in human macrophages and protozoa. We screened a L. pneumophila mini-Tn10 transposon library for mutants, which fail to inhibit the fusion of LCVs with lysosomes by loading of the lysosomal compartment with colloidal iron dextran, mechanical lysis of infected host cells, and magnetic isolation of LCVs that have fused with lysosomes. In silico analysis of the mutated genes, D. discoideum plaque assays and infection… CONTINUE READING
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