Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids

@article{Rodrigues2000PolyhydroxyalkanoateAI,
  title={Polyhydroxyalkanoate accumulation in Burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids},
  author={Maria Filomena de Andrade Rodrigues and Henry E. Valentin and Pierre A Berger and Minhtien Tran and Jawed Asrar and Kenneth J. Gruys and Alexander Steinb{\"u}chel},
  journal={Applied Microbiology and Biotechnology},
  year={2000},
  volume={53},
  pages={453-460}
}
Burkholderia sp. accumulates polyhydroxyalkanoates (PHAs) containing 3-hydroxybutyrate and 3-hydroxy-4-pentenoic acid when grown on mineral media under limited phosphate or nitrogen, and using sucrose or gluconate as a carbon and energy source. Solvent fractionation and NMR spectroscopic characterization of these polyesters revealed the simultaneous accumulation of two homopolyesters rather than a co-polyester with random sequence distribution of the monomers [Valentin HE, Berger PA, Gruys KJ… CONTINUE READING