Polyethyleneimine-mediated transfection of cultured postmitotic neurons from rat sympathetic ganglia and adult human retina

@article{Horbinski2000PolyethyleneiminemediatedTO,
  title={Polyethyleneimine-mediated transfection of cultured postmitotic neurons from rat sympathetic ganglia and adult human retina},
  author={Craig Horbinski and Michal K. Stachowiak and Dennis Higgins and Sarah G Finnegan},
  journal={BMC Neuroscience},
  year={2000},
  volume={2},
  pages={2 - 2}
}
Chemical methods of transfection that have proven successful with cell lines often do not work with primary cultures of neurons. Recent data, however, suggest that linear polymers of the cation polyethyleneimine (PEI) can facilitate the uptake of nucleic acids by neurons. Consequently, we examined the ability of a commercial PEI preparation to allow the introduction of foreign genes into postmitotic mammalian neurons. Sympathetic neurons were obtained from perinatal rat pups and maintained for… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 22 extracted citations

Non-viral retinal gene therapy: a review.

Clinical & experimental ophthalmology • 2012
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-10 of 23 references

Tissue culture of autonomic neurons

D Higgins, P Lein, D Osterhout, MI Johnson
Edited by Banker G and Goslin K • 1991
View 5 Excerpts
Highly Influenced

Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery.

Proceedings of the National Academy of Sciences of the United States of America • 1999
View 4 Excerpts
Highly Influenced

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Proceedings of the National Academy of Sciences of the United States of America • 1995
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…