Polyethylene glycol conjugation of recombinant methioninase for cancer therapy.

  title={Polyethylene glycol conjugation of recombinant methioninase for cancer therapy.},
  author={Yingmeei Tan and Xinghua Sun and Mingxu Xu and Z. G. An and Xuezhong Tan and Qinghong Han and Dusan A Miljkovi{\'c} and M Yang and Robert M Hoffman},
  journal={Protein expression and purification},
  volume={12 1},
Recombinant methioninase (rMETase) is a homotetrameric pyridoxal 5'-phosphate enzyme of 172-kda molecular mass derived from Pseudomonas putida and cloned in Escherichia coli. rMETase has been found previously to be an effective, anti-tumor agent in vitro and in vivo. The enzyme targets the elevated minimal methionine requirement seen in all tumor types. In order to prevent immunological reactions which might be produced by multiple dosing of rMETase and to prolong the serum half-life of rMETase… CONTINUE READING