Polyethylene-based high-performance liquid chromatography of chloroplast pigments: resolution of mono- and divinyl chlorophyllides and other pigment mixtures.

@article{Shioi1987PolyethylenebasedHL,
  title={Polyethylene-based high-performance liquid chromatography of chloroplast pigments: resolution of mono- and divinyl chlorophyllides and other pigment mixtures.},
  author={Yuzo Shioi and Samuel I. Beale},
  journal={Analytical biochemistry},
  year={1987},
  volume={162 2},
  pages={493-9}
}
In addition to most chlorophylls and their derivatives, monovinyl and divinyl chlorophyll species were separated by high-performance liquid chromatography, using a polyethylene column and a simple elution with aqueous acetone. Peak retention and resolution of the pigment separation were greatly increased by increasing the polarity of the mobile phase and… CONTINUE READING