Polyethylene Glycol Enhanced Protein Refolding

  title={Polyethylene Glycol Enhanced Protein Refolding},
  author={Jeffrey L. Cleland and Stuart E. Builder and James R. Swartz and Marjorie Winkler and Judy Y. Chang and Daniel I. C. Wang},
Previous studies on the refolding of recombinant bovine carbonic anhydrase B (CAB) indicated that polyethylene glycol (PEG) significantly enhanced the recovery of active protein by reducing aggregation. To further test the ability of PEG to enhance refolding, three recombinant human proteins, deoxyribonuclease (rhDNAse), tissue plasminogen activator (rhtPA), and interferon–gamma (rhIFN–γ) were refolded in the presence of PEG (3350 MW). rhDNAse produced from CHO cells was denatured in 7.2 M urea… CONTINUE READING