Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction

@article{Lssl2003PolyesterSI,
  title={Polyester synthesis in transplastomic tobacco (Nicotiana tabacum L.): significant contents of polyhydroxybutyrate are associated with growth reduction},
  author={Andreas G{\"u}nter L{\"o}ssl and Christian Eibl and H J Harloff and Choonkyun Jung and H. M. Koop},
  journal={Plant Cell Reports},
  year={2003},
  volume={21},
  pages={891-899}
}
The pathway for synthesis of polyhydroxybutyrate (PHB), a polyester produced by three bacterial enzymes, was transferred to the tobacco plastid genome by the biolistic transformation method. The polycistronic phb operon encoding this biosynthetic pathway was cloned into plastome transformation vectors. Following selection and regeneration, the content and structure of plant-produced hydroxybutyrate was analysed by gas chromatography. Significant PHB synthesis was limited to the early stages of… CONTINUE READING