Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD

@inproceedings{AndradeRodrguez2004PolyembryonyAI,
  title={Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD},
  author={Mar{\'i}a Andrade-Rodr{\'i}guez and {\'A}ngel Villegas-Monter and Guillermo Mart{\'i}n Carrillo-Casta{\~n}eda and Armando Garc{\'i}a-Vel{\'a}zquez},
  year={2004}
}
The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitro for six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P≤0.05) the morphological characteristics of… CONTINUE READING

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