Polyelectrolyte-mediated increase of biofilm mass formation

Abstract

Biofilm formation is associated with various aspects of bacterial and fungal infection. This study was designed to assess the impact of diverse natural polyelectrolytes, such as DNA, F-actin, neurofilaments (NFs), vimentin and purified Pf1 bacteriophage on biofilm formation and swarming motility of select pathogens including Pseudomonas aeruginosa associated with lung infections in CF patients. The bacteriophage Pf1 (1 mg/ml) significantly increased biofilm mass produced by Pseudomonas aeruginosa P14, Escherichia coli RS218 and Bacillus subtilis ATCC6051. DNA, F-actin, NFs and Pf1 also increased biofilm mass of the fungal C. albicans 1409 strain. Addition of F-actin, DNA or Pf1 bacteriophage to 0.5% agar plates increased swarming motility of Pseudomonas aeruginosa Xen5. The presence of polyelectrolytes at infection sites is likely to promote biofilm growth and bacterial swarming.

DOI: 10.1186/s12866-015-0457-x

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@inproceedings{Bucki2015PolyelectrolytemediatedIO, title={Polyelectrolyte-mediated increase of biofilm mass formation}, author={Robert Bucki and Katarzyna Niemirowicz and Urszula Wnorowska and Marzena Wątek and Fitzroy J. Byfield and Katrina Cruz and Marta M Wr{\'o}blewska and Paul A. Janmey}, booktitle={BMC Microbiology}, year={2015} }