Polyelectrolyte charge corrected molecular weight and effective charge by sedimentation.

@article{Braswell1987PolyelectrolyteCC,
  title={Polyelectrolyte charge corrected molecular weight and effective charge by sedimentation.},
  author={Emory H. Braswell},
  journal={Biophysical journal},
  year={1987},
  volume={51 2},
  pages={273-81}
}
Ionic charge on a macromolecule complicates the determination of its molecular weight in solution due to the Donnan effect. Compensation for it can be made if one knows the value of the effective charge, which can be found by dialysis equilibrium across a semipermeable membrane. A moving boundary of molecules sedimenting in a centrifugal field can act as a membrane, obviating some of the disadvantages (such as selective adsorption) of a real membrane. Interference optics are used to monitor the… CONTINUE READING