Polydispersity of cartilage proteoglycans. Structural variations with size and buoyant density of the molecules.

@article{Heinegrd1977PolydispersityOC,
  title={Polydispersity of cartilage proteoglycans. Structural variations with size and buoyant density of the molecules.},
  author={Dick Heineg{\aa}rd},
  journal={The Journal of biological chemistry},
  year={1977},
  volume={252 6},
  pages={1980-9}
}
Proteoglycan monomers were subfractionated according to buoyant density by dissociative CsCl density gradient centrifugation. It was shown that with decreasing buoyant density of the proteoglycan subfraction: (a) the average sizes of the molecules decreased (shown by Sepharose 2B chromatography); (b) the relative content of chondroitin sulfate decreased; (c) the relative content of protein increased; (d) the relative proportion of the amino acids glycine and serine, which occur close to the… CONTINUE READING