Polyclonal activation of canine B lymphocytes evaluated by a protein A reverse hemolytic plaque assay.

Abstract

This paper describes the optimal culture and assay conditions for the polyclonal activation of canine lymphocytes with pokeweed mitogen and the quantitation of immunoglobulin secreting plaque-forming cells (PFC) using a staphylococcal protein A-reverse hemolytic plaque assay. The assay permits the quantitation of total immunoglobulin secreting PFC as well… (More)

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@article{Felsburg1984PolyclonalAO, title={Polyclonal activation of canine B lymphocytes evaluated by a protein A reverse hemolytic plaque assay.}, author={Peter John Felsburg and Daniele Serra and V N Mandato and Peter F. Jezyk}, journal={Veterinary immunology and immunopathology}, year={1984}, volume={6 3-4}, pages={353-64} }