Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins.

@article{Zhang2013PolyacrylamideBL,
  title={Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins.},
  author={Zhaorui Zhang and Zhen Wu and Mary J Wirth},
  journal={Journal of chromatography. A},
  year={2013},
  volume={1301},
  pages={156-61}
}
A chromatographic column of nonporous silica particles with a bonded phase of linear polyacrylamide chains is evaluated for hydrophilic interaction liquid chromatography (HILIC) of intact glycoproteins. The column is shown to retain glycoproteins significantly more strongly than non-glycoproteins. A particle diameter of 700nm gives two-fold higher resolution than does a 1.4μm particle diameter, and the column efficiency is found to be mostly limited by packing heterogeneity. LCMS is able to… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-7 of 7 extracted citations

References

Publications referenced by this paper.
Showing 1-5 of 5 references

Chem

  • J. Zaia
  • Biol. 15
  • 2008
Highly Influential
3 Excerpts

Mol

  • S. Pan, R. Chen, R. Aebersold, T. A. Brentnall
  • Cell. Proteomics – MCP 10
  • 2011

L

  • Z. Tian, R. Zhao, +4 authors R. D. Smith
  • Pasa-Tolic, Proteomics 10
  • 2010
2 Excerpts

Biochim

  • R. Apweiler, H. Hermjakob, N. Sharon
  • Biophys. Acta 1473
  • 1999
2 Excerpts

J

  • J. C. Linden, C. L. Lawhead
  • Chromatogr. 105
  • 1975
2 Excerpts

Similar Papers

Loading similar papers…