Poly(amidoamine)s as potential nonviral vectors: ability to form interpolyelectrolyte complexes and to mediate transfection in vitro.

  title={Poly(amidoamine)s as potential nonviral vectors: ability to form interpolyelectrolyte complexes and to mediate transfection in vitro.},
  author={Simon C. W. Richardson and Nicola G. Pattrick and Yan Man and Paolo Ferruti and Ruth Duncan},
  volume={2 3},
Poly(amidoamine)s (PAAs) are water-soluble polymers that display pH-dependent membrane activity. PAAs have the potential to act as a synthetic alternative to fusogenic peptides and thus promote endosomal escape. The purpose of this study was to investigate for the first time whether PAA have the ability to complex DNA, protect it from nuclease degradation and to promote transfection in vitro. PAAs ISA 1 (Mn 6900) and ISA 23 (Mn 10,500) and their 2-phenylethylamine containing analogues ISA 4 and… Expand
Poly(amidoamine)-mediated intracytoplasmic delivery of ricin A-chain and gelonin.
Observations suggest that specific PAA-toxin combinations warrant further development as novel therapeutics and that the neutral polymer dextran and the PAA ISA 22 were unable to mediate this effect. Expand
Synthesis and preliminary evaluation of poly(amidoamine)-melittin conjugates as endosomolytic polymers and/or potential anticancer therapeutics.
PAA-MLT conjugates containing the membrane disrupting peptide melittin could have potential as a novel polymeric anticancer conjugate due to its lack of haemolytic activity at pH 7.4 and retention of cytotoxicity. Expand
Synthesis and endosomolytic properties of poly(amidoamine) block copolymers.
The aim of this study was to synthesise random and block copolymers of ISA 1 and ISA 23 and investigate whether these second generation hybrids would allow optimisation of PAA biological characteristics. Expand
Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties.
  • M. Piest, Chao Lin, +4 authors J. Engbersen
  • Chemistry, Medicine
  • Journal of controlled release : official journal of the Controlled Release Society
  • 2008
DMC-based SS-PAAs have very promising properties for the development of potent and non-toxic polymeric gene carriers and are capable to transfect COS-7 cells in vitro with transfection efficiencies up to 4 times higher than those of pDMAEMA and PEI. Expand
Functionalized linear poly(amidoamine)s are efficient vectors for intracellular protein delivery.
Results indicate that these poly(amidoamine)s have excellent properties as highly potent and non-toxic intracellular protein carriers, which should create opportunities for novel applications in protein delivery. Expand
Comparison of Gene Transfection and Cytotoxicity Mechanisms of Linear Poly(amidoamine) and Branched Poly(ethyleneimine) Polyplexes
PAA polyplexes displayed a pH-dependent endo/lysosomal escape which was not associated with cytotoxic events, unlike observed with BPEI polyplexa, due to their greater interactions with biological membranes at acidic than neutral pH. Expand
Intracellular fate of bioresponsive poly(amidoamine)s in vitro and in vivo.
Results provide direct evidence, for the first time, of PAA permeabilisation of endocytic vesicular membranes in vivo, and they have important implications for potential efficacy/toxicity of such polymeric vectors. Expand
Optimization of poly(amido amine)s as vectors for siRNA delivery.
It was observed that at least 20-30% EDA or TETA amino units in the copolymers is necessary to encapsulate siRNA into small and stable polyplexes (< 200 nm), which resulted in increased cytotoxicity and hemolytic activity. Expand
Effects of branched or linear architecture of bioreducible poly(amido amine)s on their in vitro gene delivery properties.
The results show the large possibilities for this class of polymers to provide polymeric vectors with controllable properties for gene therapy applications and the ease of synthetic modification of both linear and hyperbranched poly(amido amide)s and the versatility of hyperbrANChed PAAs in regulating DNA transfection and cytotoxicity. Expand
Poly(amidoamine)s: Past, present, and perspectives
Poly(amidoamine)s (PAAs) are a family of synthetic polymers obtained by stepwise polyaddition of prim- or sec-amines to bisacrylamides. Nearly all conceivable bisacrylamides and prim- or sec-aminesExpand