Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp. ND199 Using Glycerol as a Carbon Source

  title={Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp. ND199 Using Glycerol as a Carbon Source},
  author={Doan Van-Thuoc and Tran Huu-Phong and Dang Minh-Khuong and Rajni Hatti-Kaul},
  journal={Applied Biochemistry and Biotechnology},
Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of… 
Yangia sp. ND199 is a halophilic bacterium isolated from m angrove soil sample. This strain was able to produce polyhydroxyalkanoate (PH A) from different carbon sources. Only homopolymer
Utilization of waste fish oil and glycerol as carbon sources for polyhydroxyalkanoate (PHA) production by Salinivibrio sp. M318.
A moderately halophilic bacterium isolated from fermenting shrimp paste, Salinivibrio sp. M318 was found capable of using fish sauce and mixtures of waste fish oil and glycerol as nitrogen and carbon
Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides
Yangia sp. ND199 is a moderately halophilic bacterium isolated from mangrove samples in Northern Vietnam, which was earlier reported to grow on several sugars and glycerol to accumulate
Valorization of waste glycerol for the production of poly (3-hydroxybutyrate) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by Cupriavidus necator and extraction in a sustainable manner.
This is the first report on jatropha based glycerol valorization for poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) production coupled with extraction using non-toxic solvent.
Improving biological production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) co-polymer: a critical review
Although poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is the most promising biopolymer for petroleum-based plastics replacement, the low processes productivity as well as the high sale price
Fed-Batch Strategies for Production of PHA Using a Native Isolate of Halomonas venusta KT832796 Strain
This is the highest amount of PHA reported for a Halomonas venusta strain, and pH-based feeding, although improved PHA level to 26 g/L, most of the carbon flux was diverted towards biomass formation; hence, the percent PHA was only 39%.
Bio-based conversion of volatile fatty acids from waste streams to polyhydroxyalkanoates using mixed microbial cultures.
A process with the potential to reduce the production cost of polyhydroxyalkanoates was proposed and the impact of two types of carbon sources was investigated by analyzing the microbial community as well as the polyhydroxalkanoate accumulation capacity.
Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources
Two bacterial strains able to produce polyhydroxyalkanoates (PHAs) from a wide variety of pure carbon sources were isolated from forest soils and identified as Achromobacter mucicolens and Stenotrophomonas rhizophila.
Genome features of moderately halophilic polyhydroxyalkanoate-producing Yangia sp. CCB-MM3
Yangia sp. CCB-MM3 was one of several halophilic bacteria isolated from soil sediment in the estuarine Matang Mangrove, Malaysia. So far, no member from the genus Yangia, a member of the
Potential Role of Halophile in Crude Glycerol Based Biorefinery
Biorefinery includes microbial fermentation processes which could utilize glycerol as raw material for the production of bio-derived building block compounds and polymers. The recent expansion in


Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture
High poly(3-hydroxybutyrate) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium and glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density.
Effect of carbon source and concentration on the molecular mass of poly(3-hydroxybutyrate) produced by Methylobacterium extorquens and Alcaligenes eutrophus
In shake-flask culture, Methylobacterium extorquens accumulated poly(3-hydroxybutyrate) (PHB) possessing a substantially higher weight-average molecular mass (MW) than previously reported for this
Poly(3-Hydroxybutyrate) Production from Glycerol by Zobellella denitrificans MW1 via High-Cell-Density Fed-Batch Fermentation and Simplified Solvent Extraction
Zobellella denitrificans MW1, a recently isolated bacterium, was used for the production of poly(3-hydroxybutyrate) (PHB) from glycerol as the sole carbon source and a simple organic solvent extraction process was employed for PHB recovery during downstream processing.
Preparation and characterization of poly(hydroxyalkanoate) from the fermentation of Haloferax mediterranei
Through observing the variation of relative intensity of these two melting peaks by changing the heating rate, it was proven that the phenomenon is caused by a melt/recrystallization process.
Production in Escherichia coli of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) with Differing Monomer Compositions from Unrelated Carbon Sources
A PHBV biosynthetic pathway from a single unrelated carbon source via threonine biosynthesis in Escherichia coli is developed, and the largest 3-hydroxyvalerate fraction obtained in the copolymer was 17.5 mol%.
Effects of Culture Conditions on Poly(β-Hydroxybutyric Acid) Production by Haloferax mediterranei
The halobacterium Haloferax mediterranei is an interesting candidate for industrial production of biological polyesters and was stable over a 3-month period.
Synthesis of short-/medium-chain-length poly(hydroxyalkanoate) blends by mixed culture fermentation of glycerol.
Glycerol was used as a substrate in the bio-production of poly(hydroxyalkanoates) (PHAs) in an effort to establish an alternative outlet for glycerol and produce value-added products and proton-NMR analysis revealed that the M(n) decrease was the result of esterification of Glycerol onto the polymer in a chain terminating position.
Growth-associated synthesis of poly(hydroxybutyric acid) in Methylobacterium rhodesianum as an expression of an internal bottleneck
Abstract Methylobacterium rhodesianum MB126 growing on fructose accumulates poly(hydroxybutyric acid) (PHB) during exponential growth in contrast to growth on methanol, where the strain accumulates
Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01.
An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80 g/L cell dry
Effect of pH on the production of bacterial polyhydroxyalkanoates by mixed cultures enriched under periodic feeding
Abstract This research investigated the effect of pH on polyhydroxyalkanoate (PHA) production by mixed cultures enriched in a sequencing batch reactor (SBR) starting from activated sludge. The