Pollen tube and root-hair tip growth is disrupted in a mutant of Arabidopsis thaliana.

Abstract

The expansion of both root hairs and pollen tubes occurs by a process known as tip growth. In this report, an Arabidopsis thaliana mutant (tip1) is described that displays defects in both root-hair and pollen-tube growth. The root hairs of the tip1 mutant plants are shorter than those of the wild-type plants and branched at their base. The tip1 pollen-tube growth defect was identified by the aberrant segregation ratio of phenotypically normal to mutant seeds in siliques from self-pollinated, heterozygous plants. Homozygous mutant seeds are not randomly distributed in the siliques, comprising only 14.4% of the total seeds, 5.3% of the seeds from the bottom half, and 2.2% of the seeds from the bottom quarter of the heterozygous siliques. Studies of pollen-tube growth in vivo showed that mutant pollen tubes grow more slowly than wild-type pollen through the transmitting tissue of wild-type flowers. Cosegregation studies indicate that the root-hair and pollen-tube defects are caused by the same genetic lesion. Based on these findings, the TIP1 gene is likely to encode a product involved in a fundamental aspect of tip growth in plant cells.

6 Figures and Tables

050100'01'03'05'07'09'11'13'15'17
Citations per Year

472 Citations

Semantic Scholar estimates that this publication has 472 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Schiefelbein1993PollenTA, title={Pollen tube and root-hair tip growth is disrupted in a mutant of Arabidopsis thaliana.}, author={John Schiefelbein and Moira E. Galway and James D . Masucci and Susannah K Ford}, journal={Plant physiology}, year={1993}, volume={103 3}, pages={979-85} }