Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).

  title={Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).},
  author={James Bert Flanegan and David Baltimore},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={74 9},
A template-dependent RNA polymerase has been isolated from poliovirus-infected cells by assaying for the ability of the enzyme to copy poly(A) complexed to an oligo(U) primer. The polymerase was solubilized with detergent, and RNA was removed by precipitation with 2 M LiCl. The solubilized polymerase required both poly(A) and oligo(U) for activity and was stimulated by Mg2+ but was inhibited by Mn2+. Poly(A)-oligo(U)-dependent poly(U) polymerase was not found in extracts of HeLa cells until… CONTINUE READING


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