Poliovirus RNA recombination: mechanistic studies in the absence of selection.

@article{Jarvis1992PoliovirusRR,
  title={Poliovirus RNA recombination: mechanistic studies in the absence of selection.},
  author={Thale Jarvis and Karla Kirkegaard},
  journal={The EMBO journal},
  year={1992},
  volume={11 8},
  pages={3135-45}
}
Direct and quantitative detection of recombinant RNA molecules by polymerase chain reaction (PCR) provides a novel method for studying recombination in RNA viruses without selection for viable progeny. The parental poliovirus strains used in this study contained polymorphic marker loci approximately 600 bases apart; both exhibited wild-type growth characteristics. We established conditions under which the amount of PCR product was linearly proportional to the amount of input template, and the… CONTINUE READING

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