Polarized secretion of the regulated secretory protein chromogranin A.

  title={Polarized secretion of the regulated secretory protein chromogranin A.},
  author={Ulrike K{\"u}hn and David V. Cohn and Sven-Ulrik Gorr},
  journal={Biochemical and biophysical research communications},
  volume={270 2},
Bovine chromogranin A (CgA), together with secreted alkaline phosphatase (SEAP) as an external control for apical secretion were expressed in MDCK cells to test if CgA contains sorting signals for polarized secretion. CgA, SEAP, and the endogenous apical marker GP80 were secreted 75-80% apically. Basolateral secretion of SEAP was inhibited 40% by ammonium chloride. Sulfate labeling and digestion with chondroitinase ABC revealed a 120 kDa proteoglycan-CgA and 75 kDa CgA. Inhibition of… CONTINUE READING

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