Polarized light microscopy in the study of the molecular structure of collagen and reticulin

  title={Polarized light microscopy in the study of the molecular structure of collagen and reticulin},
  author={Moshe Wolman and Frederick H. Kasten},
SummaryAlthough collagen structure has been studied by polarized light microscopy since the early 19th century and continued since, modern studies and reviews failed to correlate the conclusions based on data obtained by the techniques with those of polarized light microscopy. Collagen I is intensely positively birefringent in respect to length of the fibres; the positive intrinsic birefringence indicates a quasi-crystalline alignment parallel to the fibre and molecule axis of the amino acid… 
Picrosirius‐Polarization Method for Collagen Fiber Detection in Tendons: A Mini‐Review
A brief review serves as a simplistic and important primer to research developments in which differential staining of collagen types by the Picrosirius‐polarization method is increasing in diverse studies of the medical field, mainly in the assessment of the morphology, spatial distribution, and content of collagen in tendons.
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Analysis using circularly polarized light has the ability to enhance histologic assessment of tissue and can provide additional insights into the composition and structure of myocardial collagen.
Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils
A broad range of imaging techniques and the different modalities of their use are discussed in order to discuss their advantages and limitations for the assessment of bone ultrastructure organization with respect to the orientation and arrangement of mineralized collagen fibrils.
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Picrosirius Red Staining: Revisiting Its Application to the Qualitative and Quantitative Assessment of Collagen Type I and Type III in Tendon
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Ultrastructure Organization of Human Trabeculae Assessed by 3D sSAXS and Relation to Bone Microarchitecture
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Computerized histomorphometric study of the splenic collagen polymorphism: A control‐tissue for polarization microscopy
The spleen may be used as a control tissue to qualitatively visualize type I and III collagen under polarization microscopy and to validate the quality of PSR staining to accurately calibrate the angle of polarization before quantitative measurements oftype I and type III collagen.
Optimization of Picrosirius red staining protocol to determine collagen fiber orientations in vaginal and uterine cervical tissues by Mueller polarized microscopy
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The tridimensional ultrastructure of native collagenous fibrils, cytochemical evidence for a carbohydrate matrix.
Molecular conformation and packing in collagen fibrils.
Collagen--proteoglycan interactions. Localization of proteoglycans in tendon by electron microscopy.
  • J. Scott
  • Biology
    The Biochemical journal
  • 1980
Proteoglycan in foetal- and adult-rat tail tendon and adult-rabbit achilles tendon was stained for electron microscopy with a cationic phthalocyanin-like dye, based on cinchomeronic acid, in a
Sulfation as a collagen-specific reaction The ultrastructure of sulfate collagen, basement membranes and reticulin fibers as shown by topo-optical staining reactions
Collagen-specific characeteristics due to sulfate esterification on the OH groups of the peptide chains of collagen provide new approach to the study of the ultrastructure of connective tissue elements in physiology and pathology.
The tridimensional structure of native collagenous fibrils, their proteinaceous filaments.
Direct observation of a transverse periodicity in collagen fibrils
It is shown that like other fibrous protein structures, collagen fibrils do possess a strong transverse periodicity as revealed by their self-convolution functions (or auto-correlation functions)10–12, and the observed periodicity is consistent with the presence of an 80 Å structural unit in collagenfibrils in vivo.
Ultrastructural distinction between reticular and collagenous fibers with an ammoniacal silver stain
Reticular and collagenous fibers stain differently when subjected to ammoniacal silver reduction and the association of reaction product with intercellular connective tissue elements was studied with the electron microscope.