Polarized distribution of nucleoside transporters in rat brain endothelial and choroid plexus epithelial cells.

@article{Redzic2005PolarizedDO,
  title={Polarized distribution of nucleoside transporters in rat brain endothelial and choroid plexus epithelial cells.},
  author={Zoran Redzic and Jean Biringer and Kay Barnes and Stephen A. Baldwin and Hameed Ali Al-Sarraf and Pieris A. Nicola and James D. Young and Carol E. Cass and Margery A. Barrand and Stephen Hladky},
  journal={Journal of neurochemistry},
  year={2005},
  volume={94 5},
  pages={1420-6}
}
This study investigated mRNA expression and protein localization of equilibrative and concentrative nucleoside transporters (ENTs, CNTs) in primary cultures of rat brain endothelial cells (RBEC) and rat choroid plexus epithelial cells (RCPEC). Reverse transcriptase PCR analysis revealed that RBEC and RCPEC contained mRNA for rENT1, rENT2 and rCNT2 and for rENT1, rENT2, rCNT2 and rCNT3, respectively. Immunoblotting of membrane fractions of RBEC, fresh RCPEC and primary cultures of RCPEC revealed… CONTINUE READING

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