Polarization of the Golgi apparatus and the microtubule-organizing center in cultured fibroblasts at the edge of an experimental wound.

@article{Kupfer1982PolarizationOT,
  title={Polarization of the Golgi apparatus and the microtubule-organizing center in cultured fibroblasts at the edge of an experimental wound.},
  author={A. Kupfer and D. Louvard and S. Singer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 8},
  pages={
          2603-7
        }
}
  • A. Kupfer, D. Louvard, S. Singer
  • Published 1982
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
We have used the technique of experimental wounding of confluent monolayers of normal fibroblasts to induce essentially unidirectional and synchronous cell movement at the edge of the wound. The intracellular location of the Golgi apparatus and the microtubule-organizing center was determined by double indirect immunofluorescence microscopy, using antibodies specific for the membranes of the Golgi apparatus and antibodies specific for tubulin, respectively. In cells at the wound edge, the… Expand
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