Polarity of axoplasmic microtubules in the olfactory nerve of the frog.

@article{Burton1981PolarityOA,
  title={Polarity of axoplasmic microtubules in the olfactory nerve of the frog.},
  author={Paul R. Burton and Jeffrey L. Paige},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1981},
  volume={78 5},
  pages={3269-73}
}
Pieces of olfactory nerve of the bullfrog were extracted in a tubulin assembly buffer medium containing detergents. With incubation at 37 degrees C in such medium containing soluble tubulin, ribbons of protofilaments are formed on the surfaces of microtubules, with the ribbons curving in a clockwise or counterclockwise direction. The direction of hooking reflects the polarity of the microtubule. In nerve pieces oriented such that cross sections could be viewed toward the perikarya of the axons… CONTINUE READING