Point mutations in HpuB enable gonococcal HpuA deletion mutants to grow on hemoglobin.

@article{Chen2002PointMI,
  title={Point mutations in HpuB enable gonococcal HpuA deletion mutants to grow on hemoglobin.},
  author={Ching-ju Chen and Dalton Mclean and Christopher Thomas and James E Anderson and P. Frederick Sparling},
  journal={Journal of bacteriology},
  year={2002},
  volume={184 2},
  pages={420-6}
}
Neisseria gonorrhoeae ordinarily requires both HpuA and HpuB to use hemoglobin (Hb) as a source of iron for growth. Deletion of HpuA resulted in reduced Hb binding and failure of growth on Hb. We identified rare Hb-utilizing colonies (Hb(+)) from an hpuA deletion mutant of FA1090, which fell into two phenotypic classes. One class of the Hb(+) revertants required expression of both TonB and HpuB for growth on Hb, while the other class required neither TonB nor HpuB. All TonB/HpuB-dependent… CONTINUE READING