Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism.

@article{Amichot2004PointMA,
  title={Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism.},
  author={Marcel Amichot and Sophie Tar{\`e}s and Alexandra Brun-Barale and Laury Arthaud and J Bride and J K Berge},
  journal={European journal of biochemistry},
  year={2004},
  volume={271 7},
  pages={1250-7}
}
Three point mutations R335S, L336V and V476L, distinguish the sequence of a cytochrome P450 CYP6A2 variant assumed to be responsible for 1,1,1-trichloro-2,2-bis-(4'-chlorophenyl)ethane (DDT) resistance in the RDDT(R) strain of Drosophila melanogaster. To determine the impact of each mutation on the function of CYP6A2, the wild-type enzyme (CYP6A2wt) of Cyp6a2 was expressed in Escherichia coli as well as three variants carrying a single mutation, the double mutant CYP6A2vSV and the triple mutant… CONTINUE READING

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