Point mutation of bacterial artificial chromosomes by ET recombination.

@article{Muyrers2000PointMO,
  title={Point mutation of bacterial artificial chromosomes by ET recombination.},
  author={Joep P P Muyrers and You-ming Zhang and Vladimir Benes and Giuseppe Testa and Wilhelm Ansorge and Adrian Francis Stewart},
  journal={EMBO reports},
  year={2000},
  volume={1 3},
  pages={239-43}
}
Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow rapid and precise engineering of these large molecules are required. Here, we describe a simple and flexible two-step approach based on ET recombination, which permits point mutations to be introduced into BACs without leaving any other residual change in the recombinant product. Introduction of other… CONTINUE READING
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