Podospora anserina hemicellulases potentiate the Trichoderma reesei secretome for saccharification of lignocellulosic biomass.

@article{Couturier2011PodosporaAH,
  title={Podospora anserina hemicellulases potentiate the Trichoderma reesei secretome for saccharification of lignocellulosic biomass.},
  author={Marie Couturier and Mireille Haon and Pedro M. Coutinho and Bernard Henrissat and Laurence Lesage-Meessen and Jean-Guy Berrin},
  journal={Applied and environmental microbiology},
  year={2011},
  volume={77 1},
  pages={237-46}
}
To improve the enzymatic hydrolysis (saccharification) of lignocellulosic biomass by Trichoderma reesei, a set of genes encoding putative polysaccharide-degrading enzymes were selected from the coprophilic fungus Podospora anserina using comparative genomics. Five hemicellulase-encoding genes were successfully cloned and expressed as secreted functional proteins in the yeast Pichia pastoris. These novel fungal CAZymes belonging to different glycoside hydrolase families (PaMan5A and PaMan26A… CONTINUE READING
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The biochemistry and structural biology of plant cell wall deconstruction

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