Pneumocystis jirovecii immunostain versus Gomori/Grocott methenamine silver stain of bronchoalveolar lavage in cell blocks: an institutional experience.

@article{Gonzalez2017PneumocystisJI,
  title={Pneumocystis jirovecii immunostain versus Gomori/Grocott methenamine silver stain of bronchoalveolar lavage in cell blocks: an institutional experience.},
  author={Abel Arnoldo Gonzalez and Diane Hamele-Bena and Teresa L. Wood and Sunilda Valladares-Silva and Patricia G. Wasserman},
  journal={Journal of the American Society of Cytopathology},
  year={2017},
  volume={6 6},
  pages={
          242-247
        }
}
INTRODUCTION Current approaches to Pneumocystis jirovecii (PCJ) screening on bronchioalveolar lavage samples (BAL) include Gomori/Grocott methenamine silver stain (GMS), toluidine blue O stain, Wright-Giemsa stain, immunofluorescent antibody stain, and polymerase chain reaction. Another method available is PCJ immunohistochemistry stain (PCJ IHC). There are no published series evaluating the efficacy of PCJ IHC in cell block preparation of BAL, we sought to compare GMS versus PCJ IHC at our… Expand
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References

SHOWING 1-10 OF 13 REFERENCES
Immunohistochemistry of Pneumocystis carinii infection.
TLDR
In 13 patients with AIDS, formalin-fixed paraffin-embedded autopsy lung and other visceral organ sections were stained using monoclonal antibody 3F6 (Dako, Santa Barbara, CA) to P. carinii, finding antibody staining was present in autopsy lung sections from non-AIDS patients with viral, fungal, or bacterial pneumonia. Expand
A clinical comparative study of polymerase chain reaction assay for diagnosis of pneumocystis pneumonia in non-AIDS patients.
TLDR
The diagnostic value of PCR assays of Pneumocystis jirovecii mitochondrial large subunits ribosomal RNA on sputum and BALF for pneumocyStis pneumonia are both high and equivalent. Expand
Immunohistochemical detection of Pneumocystis carinii in transbronchial lung biopsy specimens: antigen difference between human and rat Pneumocystis carinii.
TLDR
The present study indicates human PC and rat PC to be antigenically different in terms of the existence of the 82 kilo-dalton (kD) antigen against which 3F6 is directed. Expand
Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods
TLDR
Four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination found only CW and GMS had positive and negative predictive values of >90%. Expand
Pneumocystis carinii carriage in immunocompromised patients with and without human immunodeficiency virus infection.
TLDR
BAL specimens from 10 immunocompetent patients with pulmonary disorders were negative for PCP by both staining and PCR assay, and P. carinii 5S rDNA was detected by PCR assay in seven HIV-infected individuals, which was significantly more frequent than for four immunosuppressed patients without HIV infection, for whom staining was negative. Expand
Diagnostic value of direct fluorescence antibody staining for detecting Pneumocystis jirovecii in expectorated sputum from patients with HIV infection.
TLDR
It is suggested that DFA staining of expectorated sputum could be a useful initial diagnostic method in HIV-infected patients with PCP. Expand
Pneumocystis colonization is highly prevalent in the autopsied lungs of the general population.
TLDR
The results strengthen the concept that immunocompetent adults develop frequent self-limited reinfections throughout life and participate in the circulation of P. jirovecii as an infective reservoir for susceptible individuals. Expand
Impact of HIV Infection Status on Interpretation of Quantitative PCR for Detection of Pneumocystis jirovecii
TLDR
Quantitative PCR appeared to be the most sensitive test to detect Pneumocystis in BAL samples, however, because of lower inocula in HIV-negative patients, different cutoffs must be used according to the HIV status to differentiate between colonized and infected patients. Expand
Pneumocystis carinii pneumonia in patients with solid tumors and lymphomas: predisposing factors and outcome.
TLDR
In this series, protracted deep lymphopenia, long hospitalization, radiotherapy and intensive chemotherapy were considered serious risk factors for developing PCP. Expand
[Immunohistochemistry diagnosis of fungal infections].
TLDR
It is shown that it is necessary to use other kinds of antibody and fungus, in order to get more details about the possible occurrence of cross-reactions and the use of new antibodies, with new standardizations is suggested. Expand
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