Pneumocystis jirovecii immunostain versus Gomori/Grocott methenamine silver stain of bronchoalveolar lavage in cell blocks: an institutional experience.

  title={Pneumocystis jirovecii immunostain versus Gomori/Grocott methenamine silver stain of bronchoalveolar lavage in cell blocks: an institutional experience.},
  author={Abel Arnoldo Gonzalez and Diane Hamele-Bena and Teresa L. Wood and Sunilda Valladares-Silva and Patricia G. Wasserman},
  journal={Journal of the American Society of Cytopathology},
  volume={6 6},

Pneumocystis jirovecii Pneumonia in Children with Hematological Malignancies: Diagnosis and Approaches to Management

Immunofluorescence testing with monoclonal antibodies followed by fluorescent microscopy and polymerase chain reaction testing of respiratory specimens have emerged as the best diagnostic methods and measurement of (1-3)-β-D-glucan in the serum has a high negative predictive value in ruling out PJP.

A practical guide for ancillary studies in pulmonary cytologic specimens

Practical guidance is provided on the use of common ancillary studies in pulmonary cytologic specimens for refining a diagnosis, for resolving a differential diagnosis or increasingly, for predictive purposes in primary lung carcinomas.

Care of Critically Ill Patients with Human Immunodeficiency Virus

Care of patients with human immunodeficiency virus (HIV) infection in the intensive care unit (ICU) has changed dramatically since the infection was first recognized in the United States in 1981. T...



Immunohistochemistry of Pneumocystis carinii infection.

In 13 patients with AIDS, formalin-fixed paraffin-embedded autopsy lung and other visceral organ sections were stained using monoclonal antibody 3F6 (Dako, Santa Barbara, CA) to P. carinii, finding antibody staining was present in autopsy lung sections from non-AIDS patients with viral, fungal, or bacterial pneumonia.

A clinical comparative study of polymerase chain reaction assay for diagnosis of pneumocystis pneumonia in non-AIDS patients.

The diagnostic value of PCR assays of Pneumocystis jirovecii mitochondrial large subunits ribosomal RNA on sputum and BALF for pneumocyStis pneumonia are both high and equivalent.

Immunohistochemical detection of Pneumocystis carinii in transbronchial lung biopsy specimens: antigen difference between human and rat Pneumocystis carinii.

The present study indicates human PC and rat PC to be antigenically different in terms of the existence of the 82 kilo-dalton (kD) antigen against which 3F6 is directed.

Detection of Pneumocystis jiroveci in Respiratory Specimens by Four Staining Methods

Four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination found only CW and GMS had positive and negative predictive values of >90%.

Pneumocystis carinii carriage in immunocompromised patients with and without human immunodeficiency virus infection.

BAL specimens from 10 immunocompetent patients with pulmonary disorders were negative for PCP by both staining and PCR assay, and P. carinii 5S rDNA was detected by PCR assay in seven HIV-infected individuals, which was significantly more frequent than for four immunosuppressed patients without HIV infection, for whom staining was negative.

Diagnostic value of direct fluorescence antibody staining for detecting Pneumocystis jirovecii in expectorated sputum from patients with HIV infection.

It is suggested that DFA staining of expectorated sputum could be a useful initial diagnostic method in HIV-infected patients with PCP.

Pneumocystis colonization is highly prevalent in the autopsied lungs of the general population.

The results strengthen the concept that immunocompetent adults develop frequent self-limited reinfections throughout life and participate in the circulation of P. jirovecii as an infective reservoir for susceptible individuals.

Impact of HIV Infection Status on Interpretation of Quantitative PCR for Detection of Pneumocystis jirovecii

Quantitative PCR appeared to be the most sensitive test to detect Pneumocystis in BAL samples, however, because of lower inocula in HIV-negative patients, different cutoffs must be used according to the HIV status to differentiate between colonized and infected patients.

Pneumocystis carinii pneumonia in patients with solid tumors and lymphomas: predisposing factors and outcome.

In this series, protracted deep lymphopenia, long hospitalization, radiotherapy and intensive chemotherapy were considered serious risk factors for developing PCP.

[Immunohistochemistry diagnosis of fungal infections].

It is shown that it is necessary to use other kinds of antibody and fungus, in order to get more details about the possible occurrence of cross-reactions and the use of new antibodies, with new standardizations is suggested.