Pneumatic dissection and storage of donor endothelial tissue for Descemet's membrane endothelial keratoplasty: a novel technique.

@article{Busin2010PneumaticDA,
  title={Pneumatic dissection and storage of donor endothelial tissue for Descemet's membrane endothelial keratoplasty: a novel technique.},
  author={Massimo Busin and Vincenzo Scorcia and Amit K. Patel and Gianni Salvalaio and Diego Ponzin},
  journal={Ophthalmology},
  year={2010},
  volume={117 8},
  pages={
          1517-20
        }
}
PURPOSE To investigate the feasibility of pneumatic dissection of donor endothelium and the effect of 7 days of storage in tissue culture medium on the endothelial cell count. DESIGN Experimental study. PARTICIPANTS We used 20 human donor corneoscleral tissues deemed unsuitable for transplantation. INTERVENTION Donor corneas were mounted on an artificial anterior chamber and the anterior stroma removed with a 300-micron microkeratome head. Air was injected into the residual donor tissue… CONTINUE READING

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