Pluripotential competence of cells associated with Nanog activity

@article{Hatano2005PluripotentialCO,
  title={Pluripotential competence of cells associated with Nanog activity},
  author={S. Hatano and M. Tada and H. Kimura and S. Yamaguchi and T. Kono and T. Nakano and H. Suemori and N. Nakatsuji and T. Tada},
  journal={Mechanisms of Development},
  year={2005},
  volume={122},
  pages={67-79}
}
Nanog is a novel pluripotential cell-specific gene that plays a crucial role in maintaining the undifferentiated state of early postimplantation embryos and embryonic stem (ES) cells. We have explored the expression pattern and function of Nanog and a Nanog-homologue, Nanog-ps1.Nanog-ps1 was mapped on Chromosome 7 and shown to be a pseudogene. Immunocytochemical analysis in vivo showed that the NANOG protein was absent in unfertilized oocytes, and was detected in cells of morula-stage embryos… Expand
Nanog expression in mouse germ cell development.
TLDR
In germ cell development, NANOG is expressed in proliferating germ cells, in which nuclear reprogramming is progressing, and Knockout experiments indicate that Nanog functions as a key player in maintaining the pluripotency of stem cells. Expand
Downregulation of NANOG Induces Differentiation of Human Embryonic Stem Cells to Extraembryonic Lineages
TLDR
The findings suggest that NANOG acts as a gatekeeper of pluripotency in human embryonic stem and carcinoma cells by preventing their differentiation to extraembryonic endoderm and trophectoderm lineages. Expand
NANOG maintains self‐renewal of primate ES cells in the absence of a feeder layer
TLDR
It is suggested that NANOG plays a crucial role in maintaining the pluripotent state of primate ES cells. Expand
Co-localization of NANOG and OCT4 in human pre-implantation embryos and in human embryonic stem cells
TLDR
It is demonstrated that whole mount in situ hybridization is amenable to localization of mRNAs in human development, as in other species. Expand
Control of ground-state pluripotency by allelic regulation of Nanog
TLDR
It is suggested that the tight regulation of Nanog dose at the chromosome level is necessary for the acquisition of ground-state pluripotency during development, and an unexpected role for allelic expression in controlling the dose of pluripOTency factors in vivo is highlighted. Expand
Generation of Nanog reporter mice that distinguish pluripotent stem cells from unipotent primordial germ cells
TLDR
A novel Nanog‐RFP Tg mouse line that can selectively tag PSCs over unipotent PGCs is generated in which expression of red fluorescent protein (RFP) is driven by a 5.2 kb Nanog promoter/enhancer region. Expand
Nanog safeguards pluripotency and mediates germline development
TLDR
By genetic deletion, it is shown that, although they are prone to differentiate, embryonic stem cells can self-renew indefinitely in the permanent absence of Nanog, and it is surmised that Nanog stabilizes embryonicstem cells in culture by resisting or reversing alternative gene expression states. Expand
Germ cell restricted expression of chick Nanog
TLDR
Genomic analysis has shown that Nanog is an amniote‐specific gene and is absent from anamniotes and invertebrates, and other pluripotency associated genes that are located in close proximity to Nanog in human and mouse are absent from the chick genome. Expand
Heterogeneities in Nanog Expression Drive Stable Commitment to Pluripotency in the Mouse Blastocyst.
TLDR
It is proposed that the rapid timescale of early mammalian embryonic development prevents fluctuations in cell fate, and this work noted an irreversible commitment to EPI/PrE lineages in vivo. Expand
Expression of NANOG and NANOGP8 in a variety of undifferentiated and differentiated human cells.
TLDR
It is concluded that eNanOG is not exclusively expressed in undifferentiated cells and that both eNANOG and NANOGP8 may function as transcription factors in a cell type-specific manner. Expand
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