Pluripotency of mesenchymal stem cells derived from adult marrow

  title={Pluripotency of mesenchymal stem cells derived from adult marrow},
  author={Yue-hua Jiang and Balkrishna N. Jahagirdar and R. Reinhardt and Robert E. Schwartz and C. Dirk Keene and Xilma R. Ortiz-Gonz{\'a}lez and Morayma Reyes and Todd R. Lenvik and Troy C Lund and Mark Blackstad and Jingbo Du and Sara Aldrich and Aaron Lisberg and Walter C. Low and David A. Largaespada and Catherine M. Verfaillie},
We report here that cells co-purifying with mesenchymal stem cells—termed here multipotent adult progenitor cells or MAPCs—differentiate, at the single cell level, not only into mesenchymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro. When injected into an early blastocyst, single MAPCs contribute to most, if not all, somatic cell types. On transplantation into a non-irradiated host, MAPCs engraft and differentiate to the haematopoietic… 

The concept of mesenchymal stem cells.

MSCs appear to constitute a heterogeneous population of undifferentiated and committed, lineage-primed cells, capable of homing upon engraftment to a number of growth microenvironments, extensive proliferation, producing large numbers of differentiated progeny, and functional tissue repair after injury.

Stem cells as bone marrow residents

The presence of stem cells with a wide spectrum of differentiation capacities in the bone marrow allows an alternative interpretation of the phenomenon of plasticity and the possibility of their switch from a canonical to a nontrivial path of differentiation.

Marrow Stromal Mesenchymal Stem Cells

This chapter summarizes the origins, isolation, characterization, cell cycle properties, and differentiation potential of bone marrow stromal mesenchymal stem cells (MSCs).

Mesenchymal stem cells: cell biology and potential use in therapy.

It is envisaged that mesenchymal stem cells can be used in systemic transplantation for generalized diseases, local implantation for local tissue defects, as a vehicle for genes in gene therapy protocols or to generate transplantable tissues and organs in tissue engineering protocols.

Unexpected Potential of Adult Stem Cells

The finding that stem cells exist in post-natal tissues with previously unknown proliferation and differentiation potential opens up the possibility of using autologous stem cells to treat a host of degenerative, traumatic or congenital diseases.

Differentiation of embryonic stem cells in adult bone marrow.

Rat bone marrow derived mesenchymal stem cells differentiate to germ cell like cells

It is demonstrated that rat bone marrow derived MSCs are able to differentiate into germ-line cells in vitro which has an enormous scope in the advancement of fertility research and could be a sustainable source of sperm / oocyte production for potential therapeutic applications in future.

Human mesenchymal stem cell culture for neural transplantation.

Methods for the reproducible culture and neural differentiation of human MSCs generated from patient marrow are described, which provide an attractive and practical source of stem cells for reparative therapy in patients.

Adult Stem Cells and Pancreatic Differentiation

There is significant evidence that specific treatments may induce insulin expression, even if doubts remain about the true nature of the end product, and MSCs have other extraordinary features that go beyond their differentiation potential, as they may provide other cells with appropriate engraftment/differentiation niches.

Non-hematopoietic bone marrow stem cells: molecular control of expansion and differentiation.




Multilineage potential of adult human mesenchymal stem cells.

Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.

Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells.

MPCs that proliferate without obvious senescence under clinically applicable conditions and differentiate at the single-cell level not only into mesenchymal cells but also cells of visceral mesoderm may be an ideal source of stem cells for treatment of genetic or degenerative disorders affecting cells of mesodermal origin.

Multilineage Differentiation from Human Embryonic Stem Cell Lines

Because they have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, human ES cells could potentially provide an unlimited supply of tissue for human transplantation.

Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

It is reported that intravenous injection of adult bone marrow cells in the FAH−/− mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver.

Embryonic stem cell lines derived from human blastocysts.

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate

Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells.

MAPCs, derived from normal human, mouse, and rat postnatal bone marrow primitive, multipotent adult progenitor cells, can differentiate into cells with morphological, phenotypic, and functional characteristics of hepatocytes, and may be an ideal cell for in vivo therapies for liver disorders or for use in bioartificial liver devices.

Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion

It is demonstrated that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3, which, without detailed genetic analysis, might be interpreted as ‘dedifferentiation’ or transdifferentiation.

Adult Bone Marrow Stromal Cells Differentiate into Neural Cells in Vitro

It is demonstrated that human and mouse BMSC can be induced to differentiate into neural cells under experimental cell culture conditions.

Changing potency by spontaneous fusion

A mechanism by which progenitor cells of the central nervous system can give rise to non-neural derivatives is defined, and it is proposed that transdetermination consequent to cell fusion could underlie many observations otherwise attributed to an intrinsic plasticity of tissue stem cells.