Ploidy doubling by in vitro culture of excised protocorms or protocorm-like bodies in Phalaenopsis species

@article{Chen2009PloidyDB,
  title={Ploidy doubling by in vitro culture of excised protocorms or protocorm-like bodies in Phalaenopsis species},
  author={Wen Huei Chen and Ching Yan Tang and Yu Kao},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2009},
  volume={98},
  pages={229-238}
}
Endopolyploidy was observed in the protocorms of diploid Phalaenopsis aphrodite subsp. formosana with ploidy doubling achieved by in vitro regeneration of excised protocorms, or protocorm-like bodies (PLBs). Thirty-four per cent of the PLBs regenerated from the first cycle of sectioned protocorms were found to be polyploids with ploidy doubled once or twice as determined by flow-cytometry. The frequency of ploidy doubling increased as the sectioning cycles increased and was highest in diploid… CONTINUE READING

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