Platform dependencies in bottom-up hydrogen/deuterium exchange mass spectrometry.

@article{Burns2013PlatformDI,
  title={Platform dependencies in bottom-up hydrogen/deuterium exchange mass spectrometry.},
  author={Kyle M Burns and M Varela Rey and Charles A. H. Baker and David C. Schriemer},
  journal={Molecular & cellular proteomics : MCP},
  year={2013},
  volume={12 2},
  pages={539-48}
}
Hydrogen-deuterium exchange mass spectrometry is an important method for protein structure-function analysis. The bottom-up approach uses protein digestion to localize deuteration to higher resolution, and the essential measurement involves centroid mass determinations on a very large set of peptides. In the course of evaluating systems for various projects, we established two (HDX-MS) platforms that consisted of a FT-MS and a high-resolution QTOF mass spectrometer, each with matched front-end… CONTINUE READING