[Platelet derived growth factor receptor β over-expression in endothelial progenitor cells promote reendothelialization after vascular injury].

Abstract

OBJECTIVE To observe the effect of platelet derived growth factor receptor β (PDGFR-β) transfected endothelial progenitor cells (EPCs) on vascular regeneration. METHODS Spleen-derived mononuclear cells (MNCs) were isolated using density gradient centrifugation and induced with special culture medium. EPCs transfection was performed with Lipofectamine(TM) 2000 reagent according to the instruction manual. Carotid artery injury was induced in splenectomized mice. EPCs were injected by tail vein immediately and at 24 h after endothelial injury of the carotid artery. Evans blue staining was performed to evaluate reendothelialization at 7 days after endothelial injury of the carotid artery. RESULTS Most adherent cells were LDL and UEA-I double positive. Laser scanning confocal microscopy showed that transfection efficiency was about 50%-60%. The reendothelialized area in the PDGFR-β-EPCs group was significantly larger than that in EGFP-EPCs group. CONCLUSION Transplantation of PDGFR-β over-expressed EPCs can promote reendothelialization in the early phase after carotid artery injury.

Cite this paper

@article{Wang2014PlateletDG, title={[Platelet derived growth factor receptor β over-expression in endothelial progenitor cells promote reendothelialization after vascular injury].}, author={Hang Wang and Hao Huang and Yangguang Yin and Mengyang Deng and Huali Kang and Lan Huang}, journal={Zhonghua xin xue guan bing za zhi}, year={2014}, volume={42 3}, pages={214-8} }